Francisella tularensis tularensis FSC033

Center: Broad

 Status: Assembly (15 contigs)

Data

Reference:

 NZ_AAYE00000000

 Name              Length %GC
 NZ_AAYE01000001.1 101124 33.65
 NZ_AAYE01000002.1 46675  32.87
 NZ_AAYE01000003.1 1600   34.25
 NZ_AAYE01000004.1 295522 31.87
 NZ_AAYE01000005.1 650364 31.73
 NZ_AAYE01000006.1 2400   37.29
 NZ_AAYE01000007.1 132212 32.96
 NZ_AAYE01000008.1 23680  31.04
 NZ_AAYE01000009.1 201    45.27      high GC%: 16S-23S rRNA(megablast)
 NZ_AAYE01000010.1 571    46.06      high GC%
 NZ_AAYE01000011.1 61231  32.30
 NZ_AAYE01000012.1 249955 31.91
 NZ_AAYE01000013.1 137017 32.24
 NZ_AAYE01000014.1 91009  32.87
 NZ_AAYE01000015.1 50644  33.42
 Total             1844205

Traces: from NCBI TA

Libraries:

 CENTER         PROJECT	STRAIN	LIB	TYPE	SIZE	STDEV	COUNT	Location
 WIBR           G907	033	G907A1	WGS	4,500	450	768	TA
 WIBR	        G907	033	G907A2	WGS	4,500	450	768	TA
 WIBR	        G907	033	G907A3	WGS	4,500	450	20,736	TA
 WIBR	        G907	033	G907A4	WGS	4,500	450	768	TA
 WIBR	        G919	033	454-410	454	.	.	278,235	TA
 StrainSubtotal                                                 301,275

 NC_006570-NZ_AAYE00000000.png Mummerplot  Francisella tularensis subsp. tularensis Schu 4, complete genome vs Francisella tularensis subsp. tularensis FSC033

Assembly

Locations:

 /fs/szasmg/Bacteria/F_tularensis_tularensis_FSC033/

2007_0807_AMOScmp

 Uses default trimming
 AMOScmp
 =>  13 scaff, 32 contigs

2007_0807_WGA

 Only WGS reads
 runCA-OBT.pl => new read clr, new library insert estimates 
 lib: G907A3 mean=4274.536 stdev=110.196
 => 12 scaff, 23 contigs

2007_0807_AMOScmp-OBT

 use  read clr, library insert estimates from 2007_0807_WGA 
 => 13 scaff, 23 contigs

2007_0809_AMOScmp-OBT

 use  read clr, library insert estimates from 2007_0807_WGA 
 include the 454 reads
 => 15 scaff, 20 contigs