Clostridium botulinum

Data sources

Sanger:

• Genome Project

• Naming Conventions

• Complete Genome

 Hall strain A (ATCC 3502)
 chromosome: 3,886,916 bp 28.24 GC%
 plasmid:      16,344 bp 26.80 GC%

 Mummerplot: Complete Genome vs Complete Genome

• 07/16/2001 Traces

 63,115 Sanger reads

 Read problems:
   no quality       : default 20 assigned to all the bases
   no mate pairing  : can be inferred from names (.p1c, .q1c => 27,331 mates); however there seem to be many errors (links from chromosome to the plasmid)
   no library info  : assumed there was only one library used
   no trimming info : almost all reads have "CONTAINED" alignments to the reference
                      CLR=1,len(read)
   there are 124 regions in the reference which are not covered by reads
   17K reads missing from Sanger ftp

• 08/30/2007 Traces

 78,975  Sanger reads

 Cbot[1-9]*.[pq][12]    68028    #article: insert sizes of 1.5–2.kb and 2.0–2.kb, 2.2–2.kb, and 2.5–4.0 kb
 CbBAC1*.s1c             305
 CbBAC4*.[pq]1c          430
 CbBAC7*.[spq]1c         474
 Cbot_ends*.[pq]1c      1604     #article: 19 kb inserts (2kb stdev) ; based on nucmer alignements: 9kb inserts (2kb stdev)
 CBOT[1-9]*.[pqw]        509     #415 primer walks
 CBOTC                   166     #all primer walks
 J*.[pqs]               7459
 Total                 78976

NCBI :

 Reads have not been submitted to TA

• Paper: Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes; Genome Res. published online May 22, 2007

The initial genome assembly was obtained from:

• 69,632 paired end sequences (giving 9.15-fold coverage) derived from four genomic shotgun libraries (all in pUC18 with insert sizes of 1.5–2.0 kb and 2.0–2.2 kb, 2.2–2.5 kb, and 2.5–4.0 kb) using dye terminator chemistry on ABI3700 automated sequencers;
• 1,604 pairedend sequences from one pBACe3.6 library with insert sizes of 15–23 kb (a clone coverage of 3.9-fold) were used as a scaffold.
• 9,343 directed sequencing reads were generated during finishing.

(Total 80,579 reads => 17,464 missing from ftp site)

Assembly

2007_0725_WGA

   create a .frg file
   runCA-OBT.pl (default params) 
   location: 2007_0725_WGA
   => 109 scaffolds, 243 contigs
   => library inser estimates mean=1840.917 stdev=866.039

2007_0801_AMOScmp-relaxed

  MINCLUSTER=30 , MAXTRIM=50
  => 2 scaffolds, 148 contigs
 CB.qc
 CB.chromo.png
 CB.plasmid.png
 CB-scaff.png

Location:

 /fs/szasmg/Bacteria/C_botulinum