Francisella tularensis

Data sources:

NCBI:

• Genome Projects
• SRA BCM
• TA
• TA FTP
• TA OUS18

Broad:

• MSC
• Data Download

Baylor:

• Microbial Genomes
• FTP

Type A:

FSC033 : Broad assembly (15 contigs)

Assemblies location:

 /fs/szasmg/Bacteria/F_tularensis_tularensis_FSC033/

Type B:

OSU18 : BCM complete

Assembly steps:

 1. Reads were downloaded from TA and formatted using tarchive2ca 
 2. There are 2 Sanger libraries for this project 
      BFTBP:58051 reads
      BFTDP:10409 reads
 3. The reads have been retrimmed using veraTrim (-T 10 -M 100 -E 500)
 4. runCA-OBT.pl has been used to assemble all the reads
 5. The library sizes were updates using the WGA estimates
 6. The WGA was aligned to the reference using nucmer; one rearrangement, one deletion and several SNP's were noticed

Assemblies location:

 /fs/szasmg/Bacteria/F_tularensis_holarctica_OSU18/

Final assembly location:

 /fs/szasmg/Bacteria/F_tularensis_holarctica_OSU18/best