Francisella tularensis

Data sources:

NCBI:

• Genome Projects
• SRA BCM
• TA
• TA FTP
• TA OUS18

Broad:

• MSC
• Data Download

Baylor:

• Microbial Genomes
• FTP

Type A:

FSC033 : Broad assembly (15 contigs)

Assemblies location:

 /fs/szasmg/Bacteria/F_tularensis_tularensis_FSC033/

Type B:

OSU18 : BCM complete

Final assembly steps:

 1. Reads were downloaded from TA and formatted using tarchive2ca 
 * NC_008369.2
 2. There are 2 Sanger libraries for this project 
      BFTBP: #reads=58051 , insert_mean=2000, insert_stdev=666 
      BFTDP: #reads=10409 , insert_mean=2000, insert_stdev=666 
 3. The reads have been retrimmed using veraTrim (-T 10 -M 100 -E 500)
 4. runCA-OBT.pl has been used to assemble all the reads
 5. The library sizes were updates using the WGA estimates
      BFTBP: insert_mean=2690.042, insert_stdev=643.126
      BFTDP: insert_mean=3675.914, insert_stdev=1225
 6. The WGA was aligned to the reference using nucmer; one rearrangement, one  
    deletion and several SNP's were noticed
 7. The reads were assembled using AMOScmp (default parameters); 2 missoriented
    read pile regions were noticed
 8. The assembly was aligned to itself; 950 bp inverted repeats were identified as 
    flanking the problem regions
 9. The 2 regions were flipped   

Final assembly location:

 /fs/szasmg/Bacteria/F_tularensis_holarctica_OSU18/best

Assembly locations:

 /fs/szasmg/Bacteria/F_tularensis_holarctica_OSU18/