Brugia malayi

Articles

• Science 2007
• Science 2007 Supplementary data

Genome Info

• 6 chromosomes: 1-5, XY
• ~ 90M, 30% GC, 32% coding, 15% repeats

Other sequences

• mitochondrion finished: 13,657 bp; 24% GC
• Wolbachia endosymbiont strain TRS from Brugia malayi strain wMel complete: 1,080,084 bp; 34%GC (New England Biolabs)
• Wolbachia endosymbiont strain wMel progress (TIGR)

Genome Project

• NCBI Genome project
• NCBI AAQA00000000 AAQA01000001-AAQA01029808

 * 26,879 good ctgs
 * 2,927 contaminants (Example: AAQA01001321)

• TIGR Genome project (TRS strain)

Traces

 Libraries:
   * 2K : bulk of the sequence @TIGR
   * 15-20 K @TIGR
   * 8,000 BAC clones @Children's Hospital Oakland Research Institute. 

• NCBI TA FTP

Trace summary:

  * all:          1,260,215 
  * TRACE_TYPE_CODE 
    * WGS:        1,258,277 
    * TRANSPOSON:     1,437 
    * PRIMER_WALK       501
  * CENTER NAME
    * TIGR:         856,624
    * JCVI          403,591

Assemblies

TIGR

• 9X coverage, 856K Sanger traces => 8,200 scaff & 29,808 ctg (avg. scaff=~10K & avg ctg=~3K)
• "scaffolds totaling ~71 Mb of data with a further ~17.5 Mb of contigs not integrated into any scaffold (orphan contigs)" (Science 2007)