Bos taurus: Difference between revisions

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* Sequence:
* Sequence:
   Contig length summary:
   Contig length summary:
  #seqs  min    max    mean    median  n50    sum
            #seqs  min    max    mean    median  n50    sum
   210657  71      840370  13709  1523    78511  2887902366
   all      210657  71      840370  13709  1523    78511  2887902366
  placed    75775  88      840370  34512  13416  88287  2615171268
  unplaced  134882  71      166670  2022    1322    1742    272731098
 


= Problems =
= Problems =
Line 62: Line 65:
== Contaminants ==
== Contaminants ==


                          #elem  min    max    mean    median  n50    sum
                              #elem  min    max    mean    median  n50    sum       #unplaced
   list.exclude_contigs   4809    316    16661  1511    1485    1514    7266315
   list.exclude_contigs(ctgs)  4809    316    16661  1511    1485    1514    7266315   4797
   list.trim_contigs(all) 19049  457    433546  4424    1204    70673  84270091
   list.trim_contigs(ctgs)     19049  457    433546  4424    1204    70673  84270091   16631
   list.trim_contigs(bad)  30279  48      2479    354    319    445    10724357
   list.trim_contigs(regions)  30279  48      2479    354    319    445    10724357
   7266315+10724357=17,990,672=18M se to trim
   7266315+10724357=17,990,672=18M bp to trim


= Local files =
= Local files =

Revision as of 14:09, 9 December 2008

BCM

NCBI

 SPECIES_CODE = "BOS TAURUS"                                                          37,788,710 traces
 SPECIES_CODE = "BOS TAURUS" and CENTER_NAME = "BCM"                                  35,596,825 traces
 
 SPECIES_CODE = "BOS TAURUS" and CENTER_NAME = "BCM" and TRACE_TYPE_CODE = "WGS"      24,863,627 traces
 SPECIES_CODE = "BOS TAURUS" and CENTER_NAME = "BCM" and TRACE_TYPE_CODE = "SHOTGUN"  10,716,306 traces
 SPECIES_CODE = "BOS TAURUS" and CENTER_NAME = "BCM" and TRACE_TYPE_CODE = "CLONEEND"     16,892 traces

Submission

  • Title: "A whole-genome assembly of the cow, Bos taurus"
  • Authors:
 Steven Salzberg
 Aleksey Zimin
 Arthur Delcher
 Liliana Florea
 David Kelley
 Finian Hanrahan
 Guillaume Marcais
 Geo Pertea
 Michael Roberts
 Michael Schatz
 Curt Van Tassell
 James Yorke
 Poorani S.
  • Assembler:
 Celera Assembler and UMD Overlapper.
  • Sequencing Center :
 Baylor College of Medicine. 
  • Source of DNA used for sequencing:

The source of the BAC library DNA was Hereford bull L1 Domino 99375, registration number 41170496. Dr. Michael MacNeil's laboratory, USDA-ARS, Miles City, MT provided the blood. The DNA for the whole genome shotgun sequences was provided by Dr. Timothy Smith's laboratory, U.S. Meat Animal Research Center, Clay Center, NE from white blood cells from L1 Dominette 01449, American Hereford Association registration number 42190680 (a daughter of L1 Domino 99375). A skin cell fibroblast cell line from the same animal is available from Dr. Carol Chitko-McKown's laboratory, although there is no sequence from that cell line.

  • Sequence modifiers:
 [organism=Bos taurus][breed=Hereford][tech=wgs][chromosome=...]
  • Submission: Use sequin
 /nfshomes/dpuiu/szdevel/sequin.8.10/sequin
  • Sequence:
 Contig length summary:
           #seqs   min     max     mean    median  n50     sum
 all       210657  71      840370  13709   1523    78511   2887902366
 placed    75775   88      840370  34512   13416   88287   2615171268
 unplaced  134882  71      166670  2022    1322    1742    272731098


Problems

Duplicates

 deg0003136509,7180003440308 : both unplaced
 deg0003084562,7180002954167 : both unplaced

Contaminants

                             #elem   min     max     mean    median  n50     sum        #unplaced
 list.exclude_contigs(ctgs)  4809    316     16661   1511    1485    1514    7266315    4797
 list.trim_contigs(ctgs)     19049   457     433546  4424    1204    70673   84270091   16631
 list.trim_contigs(regions)  30279   48      2479    354     319     445     10724357
 7266315+10724357=17,990,672=18M bp to trim

Local files

  • Freeze dir files
 /fs/szasmg3/bos_taurus/Bos_taurus_UMD_2.0/contigs.unplaced.fa  : sequences
 /fs/szasmg3/bos_taurus/Bos_taurus_UMD_2.0/bos_taurus.agp       : all scaffolds
  • Files uploaded
 Ftp server: ftp-private.ncbi.nlm.nih.gov
 Account: cbcb_trc
 Dir: uploads/
 Local files: /fs/szasmg3/dpuiu/bos_taurus/submission/ftp/   : 22 *sqn + 1 agp