Dpuiu definitions

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 outcome           +    -
 +                 TP   FP
 -                 FN   TN
 FP: Type I error (P value)
 FN: Type II error
 try to minimize FP => increase specificity 
 Sensitive: align as many reads as possible
 Specific:  avoid misalignments

  • Q20: the number of bases in a read that have a quality value greater than 20; quality value of 20, there is a 1 in 100 chance that the base has been misidentified