Difference between revisions of "Francisella tularensis"

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Line 54: Line 54:
 
* ~dpuiu/Data/F_tularensis/
 
* ~dpuiu/Data/F_tularensis/
 
* /fs/szasmg/Bacteria/F_tularensis
 
* /fs/szasmg/Bacteria/F_tularensis
 +
 +
== 454 ==
 +
Paired End reads:
 +
  * approximately 84-nucleotide DNA fragments
 +
  * have a 44-mer adaptor sequence in the middle
 +
  * flanked by a 20-mer sequence on each side.
 +
  * The two flanking 20-mers are segments of DNA that were originally located approximately 2.5 kb apart in the genome of interest. 
 +
  * The ordering and orienting of contigs generates scaffolds which provide a high-quality draft sequence of the genome.
 +
  [http://www.454.com/downloads/protocols/1_paired_end.pdf pair read article]

Revision as of 14:27, 23 August 2007

Data Sources

NCBI:

Broad:

Baylor:

JGI:

Read Counts

Type A

  • SCHU_S4 SDRA , Complete (1,892,819 bp)
  • FSC033 Broad, Assembly (15 contigs) ***
  • ATCC6223 Baylor (88 contigs) ***

Type B

  • OSU18 Baylor, Complete (1,895,727 bp) ***
  • KO97 Baylor (126 contigs) ***
  • MI001730 Baylor (118 contigs) ***
  • OR960246 Baylor (111 contigs) ***
  • RC503 Baylor (115 contigs)
  • 275 Broad (31 contigs) ***
  • FSC022 Broad (19 contigs) ***

Novicida

  • U112 Univ. of Washington, Complete 1,910,031 bp
  • GA99-3548 Broad (18 contigs) ***
  • GA99-3549 Broad (15 contigs) ***

Mediasiatica

File Locations on Disk

  • /fs/szdata//ncbi/genomes/Bacteria/Francisella_tularensis
  • ~dpuiu/Data/F_tularensis/
  • /fs/szasmg/Bacteria/F_tularensis

454

Paired End reads:

 * approximately 84-nucleotide DNA fragments 
 * have a 44-mer adaptor sequence in the middle 
 * flanked by a 20-mer sequence on each side. 
 * The two flanking 20-mers are segments of DNA that were originally located approximately 2.5 kb apart in the genome of interest.  
 * The ordering and orienting of contigs generates scaffolds which provide a high-quality draft sequence of the genome.
 pair read article