Francisella tularensis holarctica 275: Difference between revisions

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Line 1: Line 1:
OLD PAGE: Error : Should be "Francisella tularensis holarctica 257" instead of "Francisella tularensis holarctica 275"
Center: Broad
Center: Broad
   Status: Assembly (31 contigs)
   Status: Assembly (31 contigs)
Line 60: Line 62:
   /fs/szasmg2/Bacteria/F_tularensis_tularensis_257
   /fs/szasmg2/Bacteria/F_tularensis_tularensis_257
   
   
'''2007_0822_WGA'''
'''2007_0822_WGA -> best'''


   Only WGS reads
   Only WGS reads
Line 71: Line 73:
   OBT        27116    64      638    971    16274337        600.17  171.69  683
   OBT        27116    64      638    971    16274337        600.17  171.69  683


   lib: G878A1 mean=4217 stdev=123 (smaller than original value)
   lib: G878A1 mean=4217 stdev=123 (smaller than original values)
   => 10 scaff, 33 contigs, 1,861,251 bp
   => 10 scaff, 33 contigs, 1,861,251 bp


'''2007_0822_AMOScmp-OBT'''
'''2007_0822_AMOScmp-OBT-454'''
   => 26 scaff, 45 contigs, 1,848,389 bp
   => 26 scaff, 45 contigs, 1,848,389 bp

Latest revision as of 21:25, 15 January 2008

OLD PAGE: Error : Should be "Francisella tularensis holarctica 257" instead of "Francisella tularensis holarctica 275"

Center: Broad

 Status: Assembly (31 contigs)

Data

Reference

 Name              Length %GC
 NZ_AAUD01000001.1 36812  31.84
 NZ_AAUD01000002.1 115617 32.08
 NZ_AAUD01000003.1 102890 32.92
 NZ_AAUD01000004.1 10327  35.19
 NZ_AAUD01000005.1 51099  32.65
 NZ_AAUD01000006.1 20543  32.96
 NZ_AAUD01000007.1 27261  32.36
 NZ_AAUD01000008.1 31779  33.38
 NZ_AAUD01000009.1 35088  32.87
 NZ_AAUD01000010.1 6394   32.48
 NZ_AAUD01000011.1 71129  32.23
 NZ_AAUD01000012.1 33752  32.51
 NZ_AAUD01000013.1 12799  30.66
 NZ_AAUD01000014.1 69654  32.39
 NZ_AAUD01000015.1 270437 31.82
 NZ_AAUD01000016.1 3155   35.06
 NZ_AAUD01000017.1 3610   30.28
 NZ_AAUD01000018.1 3425   32.26
 NZ_AAUD01000019.1 3601   34.35
 NZ_AAUD01000020.1 6135   29.85
 NZ_AAUD01000021.1 7998   32.42
 NZ_AAUD01000022.1 6110   33.16
 NZ_AAUD01000023.1 3321   31.95
 NZ_AAUD01000024.1 245186 31.03
 NZ_AAUD01000025.1 141312 30.64
 NZ_AAUD01000026.1 103447 31.73
 NZ_AAUD01000027.1 242782 32.10
 NZ_AAUD01000028.1 67966  32.93
 NZ_AAUD01000029.1 57895  33.98
 NZ_AAUD01000030.1 77546  34.12
 NZ_AAUD01000031.1 17171  32.39
 Total             1886241

Libraries:

 CENTER  PROJECT     STRAIN   LIB    TYPE SIZE  STDEV  COUNT   LOCATION
 WIBR    G878        275      G878A1  WGS 4,500  450   23,808  TA
 WIBR    G878        275      G878A2  WGS 4,500  450   768     TA
 WIBR    G878        275      G878A3  WGS 4,500  450   768     TA
 WIBR    G878        275      G878A4  WGS 4,500  450   768     TA
 WIBR    G878        275      G878A5  WGS 4,500  450   768     TA
 WIBR    G878        275      G878A9  WGS 10,000 1,000 768     TA
 WIBR    G878        275      G878A10 WGS 10,000 1,000 768     TA
 WIBR    G878        275      G878A11 WGS 10,000 1,000 768     TA
 WIBR    G878        275      G878A12 WGS 10,000 1,000 768     TA
 WIBR    R95...      257      454-255 454  .     .     901,724 TA
 Total                                                 931,676

Assembly

Location

 /fs/szasmg2/Bacteria/F_tularensis_tularensis_257

2007_0822_WGA -> best

 Only WGS reads
 runCA-OBT.pl => new read clr, new library insert estimates 
 clr got shorter by runing OBT (usually is the opposite)
 .           #traces  min     median  max     sum             mean    stdev   n50
 CLIPPING    29397    0       908     1116    25713268        874.69  137.9   916
 OBT         27116    64      638     971     16274337        600.17  171.69  683
 lib: G878A1 mean=4217 stdev=123 (smaller than original values)
 => 10 scaff, 33 contigs, 1,861,251 bp

2007_0822_AMOScmp-OBT-454

 => 26 scaff, 45 contigs, 1,848,389 bp