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== Solexa/Illumina == | == Solexa/Illumina == | ||
Links: | |||
[ftp://ftp.sanger.ac.uk/pub/PRODUCTION_SOFTWARE/data_sets/suis_solexa/ Strep suis Solexa data set for download at Sanger] | |||
[http://minds.nuim.ie/~tkeane/publications/ismb2007Poster.pdf ismb2007Poster.pdf] | |||
[http://www.sb-roscoff.fr/SG/IMG/pdf/Smith_Rennes_2007.pdf Smith_Rennes_2007.pdf] | |||
Revision as of 14:52, 17 October 2007
Sanger
454 (single reads)
454 (paired ends)
Features:
* approximately 84-nucleotide DNA fragments * have a ~ 44-mer linker sequence in the middle * flanked by a ~ 20-mer sequence on each side. * The two flanking 20-mers are segments of DNA that were originally located approximately 2.5 kb apart in the genome of interest. * The ordering and orienting of contigs generates scaffolds which provide a high-quality draft sequence of the genome.
Anomalies:
* the linker can appear (tandem,completely/partially) more than once
Links:
1_paired_end.pdf
Solexa/Illumina
Links:
Strep suis Solexa data set for download at Sanger ismb2007Poster.pdf Smith_Rennes_2007.pdf