Trace formatting

454 (paired ends)

Features:

 * approximately 84-nucleotide DNA fragments 
 * have a ~ 44-mer linker sequence in the middle 
 * flanked by a ~ 20-mer sequence on each side. 
 * The two flanking 20-mers are segments of DNA that were originally located approximately 2.5 kb apart in the genome of interest.  
 * The ordering and orienting of contigs generates scaffolds which provide a high-quality draft sequence of the genome.

Anomalies:

 * the linker can appear (tandem,completely/partially) more than once

Links:

 1_paired_end.pdf

Solexa/Illumina

Links:

 Strep suis Solexa data set for download at Sanger
 ismb2007Poster.pdf
 Smith_Rennes_2007.pdf